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American Society of Microbiology ABMM Sample Questions:

1. Microscopic examination of a Calcofluor white stained sputum sample from an immunocompromised patient with suspected invasive pulmonary infection reveals septate hyphae that branch dichotomously at acute (approximately 45°) angles. Conidial structures are observed, showing conidiophores arising from a specialized foot cell, terminating in a flask-shaped vesicle that bears phialides only on the upper two-thirds (uniseriate arrangement). Chains of small, round conidia are produced. This morphology is most characteristic of:

A) Aspergillus flavus
B) Aspergillus fumigatus
C) Rhizopus arrhizus
D) Fusarium solani


2. According to the current CDC-recommended laboratory algorithm for diagnosing HIV infection in adults and children aged >18 months, what is the recommended initial screening test?

A) HIV-1/HIV-2 antibody differentiation immunoassay
B) HIV RNA nucleic acid test (NAT)
C) HIV-1 p24 antigen assay only
D) HIV-1/HIV-2 antigen/antibody combination immunoassay


3. A patient develops a severe diarrheal illness after returning from a backpacking trip where they drank untreated stream water. Stool examination reveals motile trophozoites with multiple flagella and a prominent ventral sucking disk. The MOST likely causative agent is:

A) Entamoeba histolytica
B) Cyclospora cayetanensis
C) Cryptosporidium parvum
D) Giardia lamblia


4. A clinical laboratory receives a cerebrospinal fluid (CSF) sample from a 65-year-old patient presenting with fever, headache, and nuchal rigidity. Gram stain reveals Gram-positive cocci in pairs and short chains. Culture on blood agar grows alpha-hemolytic colonies that are optochin-susceptible and bile soluble. Which organism is most likely identified?

A) Staphylococcus aureus
B) Streptococcus agalactiae
C) Streptococcus pneumoniae
D) Enterococcus faecalis


5. Which of the following organisms is an obligate intracellular bacterium transmitted by arthropod vectors and is a common cause of Rocky Mountain Spotted Fever?

A) Coxiella burnetii
B) Borrelia burgdorferi
C) Chlamydia trachomatis
D) Rickettsia rickettsii


Solutions:

Question # 1
Answer: B
Question # 2
Answer: D
Question # 3
Answer: D
Question # 4
Answer: C
Question # 5
Answer: D

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